Fig 5.

Acute effect of psychological distress on females: energy reduction in prefrontal cortex (PFC) without any effect on substantia nigra (SN). (a) Parameters of oxygen consumption rates (OCRs) and Extracellular acidification rates (ECARs) in the PFC in (b) and in the SN. The oxygraphs of compiled data obtain per group (n = 5 rats) are shown on the top for OCRs (left oxygraph) and the ECARs (right oxygraph) in (a) and (b). The means ± SEM of the bioenergetics parameters that were graphed include: Basal Respiration, Non-mitochondrial Oxygen Consumption, Spare Respiratory Capacity, Maximal Respiration, H+ (Proton) Leak, ATP Production, Basal Glycolysis, Glycolytic Capacity. For each mitochondrial stress assay the following concentrations of reagents were used: 25 μM Oligomycin, 15 μM FCCP, 20 μM Antimycin, 20 μM Rotenone; n = 5 per group. Data are expressed as mean ± SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, two-way ANOVA with Bonferronís multiple comparisons test.