Table 1.

Psychological distress alters antioxidant proteins and neurotrophic factor expression in prefrontal cortex, midbrain, hippocampus and striatum in male and female WT and PINK1-KO rats (acute effect). Females seem to be less affected by stress than males. It was analyzed: cytosolic superoxide dismutase (SOD1), mitochondrial superoxide dismutase (SOD2), catalase (CAT), DJ-1, mitochondria content (TOM20), brain-derived neurotrophic factor (BDNF) in mature form (mBDNF) and the ratio between mBDNF and the precursor of BDNF (proBDNF), mBDNF/proBDNF. S- represents the unstressed group and S+ represents the stressed group. Data were obtained by Western blot and normalized using WT group without stress (WT S-) as a unit. n = 5 per group. Data are expressed as mean ± SD.

	MALES				FEMALES
	WT		PINK 1 KO		WT		PINK 1 KO
	S−	S+	S−	S+	S−	S+	S−	S+
Prefrontal cortex
SOD1	1	1.06 ± 0.26	0.98 ± 0.13	1.06 ± 0.11	1	0.95 ± 0.19	0.97 ± 0.33	0.76 ± 0.24
SOD2	1	1.16 ± 0.18 *	1.02 ± 0.12	1.07 ± 0.11	1	0.72 ± 0.1 ***	0.65 ± 0.15 ****	0.54 ± 0.08
CAT	1	1.16 ± 0.12	1.14 ± 0.2	1.38 ± 0.18 †	1	0.8 ± 0.26	0.53 ± 0.14 **	0.77 ± 0.26
DJ1	1	0.81 ± 0.17 *	0.87 ± 0.16	0.91 ± 0.09	1	0.98 ± 0.16	0.95 ± 0.23	0.9 ± 0.16
TOM20	1	0.79 ± 0.17 *	0.96 ± 0.15	0.99 ± 0.07	1	0.8 ± 0.21	0.88 ± 0.19	0.84 ± 0.23
mBDNF	1	0.8 ± 0.39	1.23 ± 0.31	1.2 ± 0.7	1	1.46 ± 0.54 *	0.85 ± 0.2	0.96 ± 0.34
mBDNF/proBDNF	1	0.75 ± 0.37	1.15 ± 0.34	1.48 ± 0.9	1	1.27 ± 0.55	0.75 ± 0.13	0.83 ± 0.24
Midbrain
SOD1	1	0.85 ± 0.29	0.77 ± 0.22	0_72 ± 0.13	1	0.87 ± 0.19	1.08 ± 0.36	1.41 ± 0.65
SOD2	1	0.92 ± 0.23	0.95 ± 0.15	0.93 ± 0.08	1	0.95 ± 0.14	0.98 ± 0.43	0.68 ± 0.15
CAT	1	0.43 ± 0.15 ****	0.48 ± 0.21 ****	0.68 ± 0.17	1	1.23 ± 0.24	0.89 ± 0.26	0.54 ± 0.151
DJ1	1	0.9 ± 0.14	0.99 ± 0.25	1.05 ± 0.31	1	1.09 ± 0.18	1.33 ± 0.53	1.13 ± 0.4
TOM20	1	0.69 ± 0.06 ***	0.76 ± 0.16 **	0.71 ± 0.13	1	0.83 ± 0.24	0.91 ± 0.28	0.75 ± 0.18
mBDNF	1	0.68 ± 0.22 **	0.79 ± 0.17 *	0.7 ± 0.15	1	0.9 ± 0.25	0.93 ± 0.45	0.61 ± 0.24
mBDNF/proBDNF	1	0.62 ± 0.18 ***	0.44 ± 0.1 ****	0.49 ± 0.12	1	0.82 ± 0.16	0.73 ± 0.35	0.55 ± 0.25
Hipocampus
SOD1	1	0.91 ± 0.21	0.99 ± 0.11	0.86 ± 0.24	1	0.92 ± 0.42	0.88 ± 0.37	0.85 ± 0.23
SOD2	1	0.98 ± 0.13	1.09 ± 0.06	0.999 ± 0.19	1	0.96 ± 0.24	0.94 ± 0.18	0.83 ± 0.16
CAT	1	1.37 ± 0.21	1.66 ± 0.46 **	1.5 ± 0.27	1	0.79 ± 0.18	1.03 ± 0.26	0.88 ± 0.2
DJ1	1	1.88 ± 0.69	2.49 ± 1.2 *	2.37 ± 1.16	1	0.9 ± 0.23	0.81 ± 0.09 *	0.66 ± 0.05
TOM20	1	1.29 ± 0.33	1.63 ± 0.33	1.76 ± 0.85	1	0.93 ± 0.34	0.94 ± 0.16	0.82 ± 0.21
mBDNF	1	1.21 ± 0.17	1.39 ± 0.29 *	1.31 ± 0.4	1	1.09 ± 0.46	0.97 ± 0.5	0.99 ± 0.76
mBDNF/proBDNF	1	1.12 ± 0.13	1.33 ± 0.2 *	1.37 ± 0.41	1	0.75 ± 0.45	0.71 ± 0.18	0.86 ± 0.32
Striatum
SOD1	1	0.95 ± 0.09	0.97 ± 0.09	0.97 ± 0.08	1	0.77 ± 0.18 **	0.73 ± 0.09 **	0.77 ± 0.17
SOD2	1	0.94 ± 0.09	0.95 ± 0.13	1.04 ± 0.08	1	0.81 ± 0.07 *	0.78 ± 0.12 *	0.84 ± 0.22
CAT	1	0.91 ± 0.07	1.12 ± 0.22	1.13 ± 0.28	1	0.74 ± 0.23	0.75 ± 0.23	0.82 ± 0.24
DJ1	1	0.999 ± 0.06	1.03 ± 0.15	1.02 ± 0.08	1	1.03 ± 0.09	0.95 ± 0.09	1.21 ± 0.37
TOM20	1	0.79 ± 0.18 *	0.91 ± 0.07	0.88 ± 0.22	1	0.8 ± 0.18	0.83 ± 0.27	0.97 ± 0.17
mBDNF	1	0.99 ± 0.44	1.01 ± 0.09	1.16 ± 0.35	1	0.85 ± 0.25	0.78 ± 0.28	0.68 ± 0.17
mBDNF/proBDNF	1	1.197 ± 0.48	0.99 ± 0.13	1.46 ± 0.64	1	0.89 ± 0.3	0.67 ± 0.14 *	0.64 ± 0.14

^*p ≤ 0.05,

^**p ≤ 0.01,

^***p ≤ 0.001,

^****p ≤ 0.0001 compared with WT S-,

^†p ≤ 0.05 compared with PINK1-KO S-, one-way ANOVA with Fisheŕs LSD test.