Figure 5:

Inhibition of SHP-1 in antitumor CD8⁺ T cells enhances cytokine production. (A) Representative flow plots show the production of IFNγ by either SHP-1 KD OT-I CD8⁺ TILs or their WT counterparts following ex vivo restimulation with full-length OVA peptide. (B) Bar graphs indicate the frequency of cytokine producing OT-I SHP-1 KD (dashed) or WT (solid) T cells at d14 post tumor cell implantation. (C) Bar graphs show the frequency of expression or MFI of surface markers CXCR5, PD-1, CD27 and Granzyme B on OT-I T cells. Error bars indicate SD. Statistical significance was determined by comparing the group means using an unpaired t test (n=8–15 mice per group, representative of three independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001.