Figure 7:

ICB combined with SHP-1 inhibition increases the frequency of IFNγ-producing endogenous antitumor T cells. (A) Bar graphs indicate the frequency of SHP-1 KD OT-Is in the CD45⁺ population with (red dashed) and without (black dashed) ICB and CXCR3 surface expression on KD OT-Is. (B) Bar graphs show the frequency of endogenous CD8⁺ T cells (Thy1.1⁻) within the CD45⁺ population in the tumor and expression of CXCR3 and Granzyme B on endogenous CD8⁺ T cells with (red dashed) or without (black dashed) ICB. (C) Bar graphs indicate the frequency of IFNγ producing endogenous CD8⁺ T cells after ex vivo restimulation with corresponding OVA(APL) peptide. Error bars indicate SD, and statistical significance was determined by an unpaired t test (n=10–15 mice per group, representative of two independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001.