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. 2020 Apr 3;10:5908. doi: 10.1038/s41598-020-62843-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miRNA markers of Johne’s Disease (JD). Differential faecal miRNA expression in response to clinical JD shows three significant miRNAs in experiments 1 and 2. ‘Positive’ (blue dots) are animals with confirmed JD, while ‘negative’ (red dots) are healthy controls. The y-axis shows log₂ relative abundance calculated by taking the negative ddCt values to indicate fold change. (A) hsa-miR-501-5p is present in lower abundance in faeces from animals with confirmed JD relative to controls. (B) hsa-miR-92a-3p is more abundant in faeces from JD positive animals compared to controls. (C) hsa-miR-658 has lower abundance in faeces from animals with JD.