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. 2020 Apr 3;10:5948. doi: 10.1038/s41598-020-62552-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Length of lag phase versus maximum rate of growth for Bacillus subtilis 168 in Belitzki minimal medium broth, supplemented with a range of stressors. Media were supplemented with: (a) betaine at 2.56 (♦), 2.13 (■), 1.71 (▲), 1.28 (×), 0.85 (−) and 0.43 M (●), and without betaine (control; +); (b) guanidine hydrochloride at 150 (♦), 125 (■), 100 (▲), 75 (×), 50 (−) and 25 nM (●), and without guanidine hydrochloride (control; +); (c) polyethylene glycol 6000 at 50 (♦), 42 (■), 33 (▲), 25 (×), 17 (−) and 8 nM (●) and without polyethylene glycol 6000 (control; +) and (d) polyethylene glycol 600 at 400 (♦), 333 (■), 267 (▲), 200 (×), 133 (−) and 67 nM (●), and without polyethylene glycol 600 (control; +). Data were obtained from Fig. S7 (see Experimental procedures), and trend lines showing a strong correlation between data are indicated by a solid black line (c,d), while lines fitted which indicated inconsistency between lag and growth rates (i.e. no correlation was observed) are shown as a dashed grey line (a,b).