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. 2020 Apr 3;10:5948. doi: 10.1038/s41598-020-62552-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Length of lag phase versus maximum rate of growth for Saccharomyces cerevisiae at 30 °C: strain 14 in salt-supplemented nutrient broth (SSNB) (a) and yeast extract peptone dextrose broth (YPDB) (b); and strain 77 in SSNB (c) and YPDB (d). Cells suspended in water (not subjected to any dehydration treatment) (pink squares), and cells suspended in 1 M xylitol (no dehydration treatment) (orange squares), were used as controls. For dehydrated cells, rehydration treatments were either rapid, using water (grey squares); rapid, using 1 M xylitol (brown squares); gradual, using water vapour then rapid in water (purple squares); or gradual rehydration with water vapor, then rapid in 1 M xylitol (turquoise squares) or for xylitol dehydrated cells; gradual, using water vapour then rapid in water (×); and gradual, using water vapour then rapid in 1 M xylitol (▲). Trend lines showing a strong correlation between data are indicated by a solid black line (d), while inconsistency between lag and growth rates (i.e. no correlation was observed) is indicated by a dashed grey line (a–c).