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. 2020 Apr 3;11:1679. doi: 10.1038/s41467-020-15408-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Photomicrograph (63 × magnification) of adrenal slice from a zG-GCaMP3 expressing mouse before Ang II stimulation. Individual cells within rosettes can be distinguished by their dark nucleus, surrounded by cytosolic GCaMP3. Scale bar: 20 µm. b Representative trace of fluorescence intensity (top) imaged in a zG cell before and after stimulation with 3 nM Ang II (red line, 90 s after start of record) and corresponding raster plot (middle) of calcium spikes captured at 20 Hz for 10 min. (bottom) Spectrogram of oscillation frequency after Fourier transformation. A magnification of the region indicated by dashed red rectangle is provided on the far right. c Raster plot of calcium spikes (left) and corresponding spectrogram of oscillation frequencies (right) from all cells measured in a single adrenal slice. Solid red line indicates when 3 nM Ang II was added to the bath solution. Scale bar: 60 s. d Frequency distribution of inter-calcium spike intervals (0.1 min bins) elicited by 3 nM Ang II is normally distributed (mean ± SEM [Hz]: 0.519 ± 0.006, n = 24,052). Source data are provided as a Source Data file.