Fig. 2. Mutation frequency is tightly correlated with effects on the cleavage rate.

Structural heatmaps of k_cat (a) and apparent K_M (b) for all single mutants and basepaired-partner double mutants. Magenta arrowheads indicate consensus ribozyme values. c Structural heatmap of mutation frequency for point mutants, and previously reported basepair covariation, from ref. ⁹ (Methods). d–f Scatterplots of mutation frequency vs. cleavage rate (d), K_M (e), and k_cat/K_M (f) for point mutants of the ribozyme core. Dotted magenta lines indicate consensus ribozyme values. g Scatterplot of K_M vs. k_cat for all variants for which both parameters were determined. Source data are available in the Source Data file.