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. 2020 Apr 3;10:5852. doi: 10.1038/s41598-020-62615-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of combination of two Na_V1.4 S4-S5_L mimicking peptides that both had an effect on Na_V1.4 current density when expressed alone. (A) representative, superimposed current recordings in COS-7 cells co-transfected with Na_V1.4, Na_Vß1, and control 1 (top trace) or the combination of domain I S4-S5_L(+3) peptide and domain III S4-S5_L(0) peptide (bottom trace). Inset: activation voltage protocol used (holding potential: −100 mV; 30-ms pulse; one sweep every 2 s). (B) Dot plot and mean ± sem of peak Na_V1.4 current densities recorded in COS-7 cells co-transfected with Na_V1.4, Na_Vß1, and the indicated peptides, at 0 mV. C: Relative peak conductance versus membrane potential curves for Na_V1.4 channels in the same cell groups as in (B). Lines are Boltzmann fits to the data. (D,E) Dot plot and mean ± sem of Na_V1.4 half-activation potential (V_0.5; D) and activation slope (K; E) in the same cells group as in (B). *p value vs. both controls <0.05.