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. 2020 Apr 3;10:5859. doi: 10.1038/s41598-020-62755-9

Figure 2.

Figure 2

The effects of Si-based agent on renal function and the impacts of oxidative stress. (a) The effects of Si-based agent on serum creatinine. (b) The effects of Si-based agent on urinary protein. (c) 8-hydroxydeoxyguanosine (8-OHdG) staining after for measuring the impact of oxidative stress. The dark brown dots (arrow) correspond to representative 8-OHdG-positive nuclei in the control group (i) or the Si-based agent (0.1%–1.0%) groups (ii-iv). Scale bar: 100 µm. We counted the number of 8-OHdG-positive cells per 100 cells in ten random areas of each rats (v). In addition, we measured the urinary 8-OHdG level with ELISA kit (vi). Data were expressed as mean +/− SD. **p < 0.01, *p < 0.05 vs. the control group, respectively.