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. 2020 Apr 3;10:5929. doi: 10.1038/s41598-020-62569-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Schematic illustration of the proposed workflow for the fabrication of inertial microfluidic devices. I. The desired channel geometry was printed by a high-resolution SLA/DLP 3D printer II. After cleaning the part by isopropanol, it was bonded to a PMMA sheet by means of a double-coated pressure-sensitive adhesive tape. The entire process just takes less than two hours. III. Benefitting from the PMMA transparency, high-speed, fluorescent, bright field, or phase contrast microscopy can be performed from the bottom side of the channel (B) An actual complicated inertial microfluidic device containing a spiral and serpentine microchannel. (C) Fluorescent microscopy from the bottom side of the channel.