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. 2020 Apr 3;10:5835. doi: 10.1038/s41598-020-62456-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterisation of EVs purified from plasma collected before and after BFRE. Using the NTA technology, the EV concentration (a) and size (b) were determined for the two purification methods, precipitation using the miRCURY Exosome Isolation Kit (0.5 ml plasma) or size exclusion chromatography (SEC) using qEVs (1 ml plasma) (mean +/− SEM, n = 6). Transmission electron microscopy (TEM) images of EVs (c), scalebar 200 nm. NTA generated size distribution of purified EVs using precipitation (d) and SEC (e). Full length Western blotting strips validating the presence of the EV markers Flotilin-1 and TSG101 (f).