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. 2020 Apr 3;10:5835. doi: 10.1038/s41598-020-62456-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Next generation sequencing of small non-coding RNAs purified from EVs. The quality score assigned to each base position after quality and adaptor trimming. (a) The read length distribution (b) shows a peak from 20–25 nucleotides. Annotation overview of reads (c), with approximately 80% of the reads mapping to miRNAs. PCA plot based on the counts of all detected miRNAs. (d) A green (pre-samples) and blue (post-samples) polygon denotes the smallest space to contain the pre and post samples, respectively. Rmsk: RepeatMasker, rfam: Database of functional non-coding RNA families.