Table 2.
EV Array analysis of plasma EV surface marker expression before and after BFRE.
| EV surface marker | % change to pre | P-Value |
|---|---|---|
| Integrin alpha 2b/CD41 | 36 | 0.00019 |
| Flotillin 1 | −24 | 0.00019 |
| NCAM | 25 | 0.0026 |
| Alix/PDCD6IP | 18 | 0.024 |
| IL2RA/CD25 | 21 | 0.049 |
| CD81 | 6 | 0.41 |
| CD63 | 5 | 0.50 |
| CD9 | 1 | 0.88 |
Antibodies against 40 surface proteins were printed on epoxy-coated glass slides and used to capture EVs present in plasma. A cocktail consisting of antibodies against the canonical EV surface proteins CD9, CD63 and CD81 coupled with fluorescent probes were used for detection. Five surface markers changed expression after BFRE. Four were up-regulated: CD41, NCAM, Alix, CD25; while one was down-regulated: Flotillin-1. No change in the canonical EV markers CD9, CD63, CD81 was observed.