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. 2020 Apr 3;11:1680. doi: 10.1038/s41467-020-15332-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Laminin β1 (Lamb1, green) marks the basement membrane surrounding distinct clusters of zG cells (Gαq+, magenta), defining the outline of individual glomerulus. b Cells within each glomerulus organize into rosettes. Representative image of an adult adrenal slice stained for Laminin β1 (Lamb1, green) and β-catenin (red). Dashed circles highlight a rosette example. Arrowheads point to rosette center. c Top, confocal z-stack images of the rosette encircled in (b), showing β-catenin (red) and nuclei (DAPI, blue). Z step size is 1 μm. Arrowhead points to the rosette center. Bottom, tracing of cells (pseudo-colored and numbered 1–12) participating in the rosette shown in top panel. c capsule, zG zona glomerulosa, zF zona fasciculata. DAPI (blue) marks nuclei. All bars, 10 μm.