Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 3;11:1658. doi: 10.1038/s41467-020-15525-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a CD3ε T cells (CD45⁺/CD11b⁻/CD3ε⁺) in limbs harvested from control and arthritic mice at ZT6 (control n = 7; CIA n = 5) and ZT18 (control n = 7; CIA, n = 8), data normalised to CD3ε T cell numbers in control limbs at ZT6, two-way ANOVA and Bonferonni post hoc tests. b Quantification of CD4⁺ T cell subsets (T_h1 and T_h17) in limbs at ZT6 (T_h1: control n = 7; CIA n = 13; T_h17: control n = 8; CIA n = 13) and ZT18 (T_h1: control n = 5; CIA n = 12; T_h17: control n = 8; CIA n = 12), data pooled from two separate experiments and normalised to ZT6 control animals, two-way ANOVA and post hoc Bonferonni. c Quantification of Tregs in limbs at ZT6 (control n = 8; CIA n = 13) and ZT18 (control n = 8; CIA n = 12), data pooled from two separate experiments and normalised to ZT6 control animals, two-way ANOVA and post hoc Bonferonni. d Expression of the proliferation markers Ki67 (ZT6: control n = 4; CIA n = 7; ZT18: control n = 4; CIA n = 13) and EdU (ZT6: control n = 6; CIA n = 8; ZT18: control n = 10; CIA n = 8) on Tregs isolated from control and inflamed joints, Kruskal–Wallis test and post-hoc Dunn’s test. e Expression of NRP1 and GITR (ZT6: control n = 4; CIA n = 6; ZT18: control n = 4; CIA n = 12) on Tregs isolated from control and inflamed joints, two-way ANOVA and post-hoc Bonferonni test. NRP1—interaction between time-of-day and disease effect (p = 0.0285); GITR—disease effect (p = 0.0077). f IL10 expression by stimulated Tregs isolated from inflamed joints at ZT6 (n = 8) and ZT18 (n = 17) expressed as total number of IL10⁺ Tregs and % of Tregs that are IL10⁺, Mann–Whitney test. g Quantification of Tregs in blood of arthritic mice (ZT6 n = 3; ZT18 n = 6), two-sided unpaired T-test. h Quantification of Tregs in inguinal (ZT6 n = 7; ZT18 n = 5) and popliteal (ZT6 n = 5; ZT18 n = 4) lymph nodes from arthritic mice. Treg numbers were determined per lymph node, only popliteal lymph nodes draining inflamed limbs were analysed, two-sided unpaired T tests. All graphs show individual data points with mean values. In all panels statistical significance between indicated groups is shown as *p < 0.05, **p < 0.01 and ***p < 0.005. Source data are provided as a Source Data file.