Table 2.

Time-response of ID1, ID3 and DLX2 expression in HSC2 cells exposed to milk.

MM	30 min	60 min	3 hours	24 hours
ID1	4.5 ± 3.9	3.6 ± 3.7	18.5 ± 22.3	11.5 ± 8.2
ID3	19.6 ± 21.6	17.7 ± 22.9	13.4 ± 8.4	16.3 ± 10.2
DLX2	2.6 ± 0.1	1.1 ± 0.8	34.8 ± 46.1	5.6 ± 1.5
CM	30 min	60 min	3 hours	24 hours
ID1	12.6 ± 14.7	35.9 ± 65.4	16.0 ± 19.7	30.9 ± 40.0
ID3	7.0 ± 7.6	19.2 ± 23.6	5.5 ± 3.4	24.1 ± 19.7
DLX2	3.5 ± 3.7	4.4 ± 3.9	16.3 ± 19.5	43.4 ± 71.1

The oral squamous cell carcinoma cell line HSC2 was exposed to 5% aqueous fractions of pasteurized human milk (MM), cow’s milk (CM), and infant formula (IF) for the indicated time period, before expression analysis of the target genes ID1, ID3 and DLX2 were performed. Data indicate the x-fold decrease compared to unstimulated control cells. Data represent three independent experiments.