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. 2020 Apr 3;10:5923. doi: 10.1038/s41598-020-62293-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A,B) Protein expression and apoptosis analysis. Western blot analysis of HCT116 and A431 cells. (A) Expression analysis of the above-mentioned proteins for HCT116 and A431 (normalized density), n > 3 error bars = SD. (B) Representative bands of the expression levels of EGFR, HSP90, HSP70, γH2AX and the housekeeping protein (loading control) beta actin 24 h after treatment. Images display cropped blots gathered with exposure times applied equally across the entire image, however different for different membrane fragments of the assessed proteins. All images are taken from membranes of the same lysates. (C,D) Flow cytometry analysis, of Annexin V expression. (C) Annexin V expression plotted as % positive cells after 48, 72 and 96 h treatment. n > 3, error bars = SD. (D) Representative image of Annexin V and DAPI analysis as fluorescence intensity (FI) for A431 cells at 48 h.