Table 1.
Summary of differentially expressed mitochondrial proteins in MDCK cells after exposure to normal glucose (NG), high glucose (HG), or osmotic control (OS).
| Spot no. | Protein name | NCBI ID | MS/MS score | %Cov | No. of distinct/total matched peptides | pI | MW (kDa) | Intensity (Mean ± SEM) | Ratio | ANOVA p value | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NG | HG | OS | HG/NG | HG/OS | OS/NG | |||||||||
| HG-specific changes (increased) | ||||||||||||||
| 181 | Tubulin beta-2A chain | gi|4507729 | 879 | 35 | 14/28 | 4.78 | 50.27 | 0.3491 ± 0.0438 | 0.5158 ± 0.0472 | 0.3048 ± 0.0652 | 1.48 | 1.69 | 0.87 | 0.0365 |
| 610 | Prelamin A/C | gi|560891942 | 1204 | 36 | 24/27 | 6.57 | 74.47 | 0.0647 ± 0.0058 | 0.1211 ± 0.0122 | 0.0898 ± 0.0074 | 1.87 | 1.35 | 1.39 | 0.0027 |
| 661 | Annexin A2 | gi|50950177 | 892 | 50 | 18/23 | 6.92 | 38.92 | 0.0653 ± 0.0068 | 0.0956 ± 0.0136 | 0.0519 ± 0.0021 | 1.46 | 1.84 | 0.79 | 0.0125 |
| Changes induced by both HG and OS (decreased) | ||||||||||||||
| 296 | Elongation factor 1-delta | gi|587020223 | 240 | 16 | 4/4 | 5.27 | 33.02 | 0.0784 ± 0.0159 | 0.0467 ± 0.0073 | 0.0301 ± 0.0058 | 0.60 | 1.55 | 0.38 | 0.0221 |
| 655 | Peptidyl-prolyl cis-trans isomerase B isoform 2 | gi|74000476 | 875 | 59 | 16/41 | 9.33 | 23.82 | 1.6213 ± 0.1433 | 0.9199 ± 0.1203 | 0.9275 ± 0.1105 | 0.57 | 0.99 | 0.57 | 0.0025 |
| 799 | ATP synthase subunit beta, mitochondrial precursor | gi|31980648 | 630 | 26 | 12/15 | 5.19 | 56.27 | 0.0779 ± 0.0138 | 0.0392 ± 0.0048 | 0.0490 ± 0.0099 | 0.50 | 0.80 | 0.63 | 0.0493 |
NCBI = National Center for Biotechnology Information.
%Cov = %Sequence coverage = (number of the matched residues/total number of residues in the entire sequence) × 100%.