Figure 2.

Time-kill assay of phage vB_KpnS_Kp13 against K. pneumoniae 533. Overnight bacterial culture was diluted (10⁵ CFU/ml) and infected with phage vB_KpnS_Kp13 at MOI of 100 (green, ▲), at MOI of 10 (blue, ●), at MOI of 1 (yellow, ◆), at MOI of 0.1 (purple, ×), at MOI of 0.01 (orange, □), at MOI of 0.001 (pink, Δ) and at MOI of 0.0001 (grey). Non-phage treated bacterial cultures (control K.p. 533, red, ■) were used as a positive control. Optical density at 600 nm was measured every hour up to 24 h. Each data point is the mean from 3 experiments. Standard deviations (±SD) are shown as vertical lines.