Fig. 3. Genes with condition-dependent fitness in two light conditions.

a Distribution of fitness score F for all sgRNAs in clusters 1–4 (color code as in Fig. 2). Fitness score indicates the degree of enrichment (positive) or depletion (negative) of an sgRNA, normalized to cell generations. Comparison between two light conditions (L100—light with 100 µmol m⁻² s⁻¹ and L300—light with 300 µmol m⁻² s⁻¹) shows sgRNAs are on average more rapidly depleted under L300 independent of number of cell generations. b Difference in fitness (ΔF) between L100 and L300, for genes with both sgRNAs in the same cluster. Differentially depleted/enriched sgRNAs indicated in red, threshold: 3 ≤ ΔF ≤ −3. c Time courses of both sgRNAs for a selection of 18 genes with high ΔF in L100 (blue) and L300 (yellow). Source data are provided as a Source Data file.