Table 1.

Selection of sgRNA clones enriched by droplet sorting.

sgRNA	Appearances	Effect on growth	Gene function
gdhA_25*	4	None	Glutamate dehydrogenase
ssr2016_62*	3	Impaired	Ferredoxin:plastoquinone reductase, cyclic electron flow
gltA_41*	3	Impaired	Citrate synthase
pntB_19	3	None	Transhydrogenase subunit
pcnB_64*	3	Impaired	Nucleotidyltransferase
serA_10*	2	Impaired	Phosphoglycerate dehydrogenase
gltB_3*	2	Impaired	Glutamate synthase
ndhD2_15	2	None	NDH-1 complex subunit, cyclic electron flow
sdhB_26*	2	None	Succinate dehydrogenase
Entry85_13	2	None	atpA (ATP synthase) asRNA
pstC_41	2	None	Phosphate transporter
slr0453_10	2	None	Phosphoketolase

Note: Mutants were identified from a CRISPRi library based on L-lactate productivity. Appearance is the number of sorting runs (out of four) where that clone had an enrichment factor of at least 2 (normalized abundance in sorted population/normalized abundance in unsorted population). Clones where growth was impaired are denoted. These clones were significantly depleted in the L300 dataset after four generations (ssr2016_62, p_adj = 1.6e⁻⁹⁴; gltA_41, p_adj = 3.1e⁻⁵³; pcnB_64, p_adj = 1.5e⁻⁵⁸; serA_10, p_adj = 3.6e⁻²¹; gltB_3, p_adj = 0.015; multiple-hypothesis adjusted p-values calculated by DESeq2). An asterisk denotes sgRNA mutants selected for reconstruction and validation. Source data are provided as a Source Data file.