Fig. 5.

A Cellular toxicity of five curcumin derivatives. (B) Influence of five curcumin derivatives treatment on the mRNA expression of M gene in H1N1-infected MDCK cells. MDCK cells were infected with 100 μL of 100TCID₅₀ influenza virus A/Font Monmouth/47(H1N1, FM1) for 2 h at 37 °C. After removing the virus-containing medium, the cells were treated with curcumin derivatives at MNLDs in DMEM respectively. Cells were harvested 24 h after infection and mRNA expression levels of M genes were detected by real-time RT-PCR. Data represent the mean ± SD of 3 separate experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 relative to virus group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 relative to mock control group. (C) Influence of curcumin derivatives treatment on neuraminidase activation in H1N1-infected MDCK cells. MDCK cells were infected with 100 μL of 100TCID₅₀ influenza virus A/Font Monmouth/47(H1N1, FM1) for 2 h at 37 °C. After removing the virus-containing medium, the cells were treated with curcumin derivatives at MNLDs in DMEM respectively. Twenty-four hours post-infection the cell supernatant was analyzed for neuraminidase activity (expressed as mU/mL units) using Neuraminidase Activity Assay Kit. Data represent the mean ± SD of 3 separate experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 relative to virus group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 relative to mock control group