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. 2020 Mar 23;2020:4061516. doi: 10.1155/2020/4061516

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Copyright © 2020 Seung-Hun Oh et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Neural induction of human ep-iPSCs into neural precursor cells. (a) Immunofluorescence staining of undifferentiated markers (OCT4, SOX2, NANOG, SSEA4, TRA-1-60, and TRA-1-81) in induced pluripotent stem cells (ep-iPSCs). (b) Fluorescent-activated cell sorting (FACS) analysis of undifferentiated markers (SSEA4 and TRA-1-60) in ep-iPSCs. (c) Polymerase chain reaction (PCR) of undifferentiated markers (OCT4, NANOG, SOX2, and LIN28) in ep-iPSCs. (d) Double immunofluorescence staining for neural precursor cell (NPC) markers (Nestin and SOX2) in ep-iPSC-NPCs. DAPI (blue) stain nuclei. (e) PCR analysis of NPC markers (Nestin and SOX2) in ep-iPSC-NPCs. Scale bars: 50 μm. ep-iPSC-NPCs: neural precursor cells differentiated from induced pluripotent stem cells.