Table 9.
Codon usage and tRNA abundance for codons suppressible with 8 nt anticodon loop tRNAs and “shifty” codons
| Codon (acid) | Codon usage (0.1 %)a | tRNA abundance (relative)b | S124 suppression with tRNA | S124 readthrough (without tRNA) |
|---|---|---|---|---|
| AGG (Arg) | 0.9 | ND | + | + |
| CCA (Pro) | 7.4 | ND | + | |
| CCC (Pro) | 3.4 | ND | + | |
| CGG (Arg) | 3.6 | Minor | + | + |
| CUA (Leu) | 2.5 | Minor | + | |
| CUC (Leu) | 9.3 | 0.30 | + | |
| UAG (amber) | ND | N/A | + | |
| UCA (Ser) | 5.6 | 0.25 | + | |
| AAA (Lys) | 38.4 | 1.00 | + | |
| CGA (Arg) | 2.6 | 0.90 | + | |
| CUU (Leu) | 9.9 | 0.30 | + | |
| GGG (Gly) | 8.6 | 0.10 | + | |
| UUU (Phe) | 16.9 | 0.35 | + | |
| AAU (Asn) | 14.8 | 0.60 | weak | |
| ACG (Thr) | 11.4 | ND | weak | |
| GGA (Gly) | 5.5 | 0.20 | weak | |
| UUC (Phe) | 19.1 | 0.35 | weak |
ND, no data. N/A, not applicable. Minor, abundance too low for accurate measurement (Inokuchi & Yamao, 1995).
a
Usage in E. coli. If all sense codons were used equally, usage would be 16.4.
b
Relative to tRNA3Leu with codon usage 56.3.