Fig. 2.

Interaction between gga-miR-30d and replication of IBV. (A) Relative quantitation RT-PCR analysis of endogenous miR-30d in HD11 cells infected with IBV (MOI of 10) for 48 h. All data were representative of three independent experiments and presented as means ± SD.*p < 0.05, **p < 0.01 (B) IBV genome N mRNA levels were determined by relative quantitative RT-PCR assay and fold changes were calculated using the 2^-ΔΔCt method after transfected with miR-30d mimics, inhibitor and their negative controls. (C) Under the same processing conditions as Fig. 2B, Virally infected cells were visualized by immunofluorescence staining of IBV N protein (green) to further confirm the results. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)