Fig. 3.

USP47 is one of the targets of gga-miR-30d. (A)The relative expression of the seven predicted genes in HD11 cells after transfected with miR-30d mimics for 24 h, measured by qRT-PCR, using β-acting as the reference gene. All data were representative of three independent experiments and presented as means ± SD.*p < 0.05, **p < 0.01 (B) The details of the molecular interactions between miR-30d and its targets. The seed sequence of gga-miR-30d is underlined and marked with a red font. The mutant sequence is USP47 3′-UTR or WWP1 3′-UTR with indicted mutated at seed sequence. (C) MiR-30d mimics or miR-30d NC mimics were co-transfected with indicated reporter constructs in HD11 cells. Firefly and Renilla luciferase activity levels were measured at 36 h post-transfection. The activity of firefly luciferase was normalized to that of Renilla luciferase.