Table 1.

Primers used for PCR amplification and sequence analysis

Primer	Sequence 5′ to 3′	Target	MRV specificity	Sense	Position	Amplicon size (bp)
L1-rv5a	gCATCCATTgTAAATgACgAGTCTg	L1	All types	+	1888–1912	416
L1-rv6a	CTTgAgATTAgCTCTAgCATCTTCTg			−	2278–2303
L1-rv7a	gCTAggCCgATATCgggAATgCAg	L1	All types	+	1930–1953	344
L1-rv8a	gTCTCACTATTCACCTTACCAgCAg			−	2249–2273
S1-R1Fb	ggAgCTCgACACAgCAAATA	S1	Type 1	+	958–977	505
S1-R1Rb	gATgATTgACCCCTTgTGC			−	1444–1462
S1-R2Fc	CTCCCgTCACggTTAATTTg	S1	Type 2	+	1047–1066	394
S1-R2Rc	gATgAgTCgCCACTgTgC			−	1423–1440
S1-R3Fd	TgggACAACTTgAgACAggA	S1	Type 3	+	338–357	326
S1-R3Rd	CTgAAgTCCACCRTTTTgWA			−	644–663
ENT-S1-R3Fd	gCTATTggTCggATggAT	S1	Type 3	+	1–18	1416
ENT-S1-R3Rd	gATgAAATgCCCCAgTgC			−	1399–1416

^aLeary et al., 2002. Primer position is referred to the L1 sequence of T1L/53 (accession: NC00_4271).

^bPrimer position is referred to the S1 sequence of T1L/53 (accession: M35963).

^cPrimer position is referred to the S1 sequence of T2J/55 (accession: M35964).

^dPrimer position is referred to the S1 sequence of T3D/55 (accession: M10262).