Skip to main content
. 2015 Oct 21;182:75–81. doi: 10.1016/j.vetmic.2015.10.019

Table 1.

Primers and probes designed for real-time PCR assays in this study.

Target virus and region Primer or probe name Sequence (5′–3′)a 5′end position
(accession no.)
Reference
GRAV (VP6) T207-F GGAGGTTCTGTAYTCATTGTCAAAA 26 (X94617) Logan et al., 2006
T234-R CCTATTCCTCCTGTTTGAAAATCAT 178 (U10031) This study
T223-ROX AAT(+C)AAA(+T)GATAG(+T)CAC(+T)ATGA 120 (U10031) This study
GCRV (VP6) T148-F CCGTGAAGAGAATGGTGATGTAGA 1187 (M94157) Chun et al., 2010
T243-R CATAGTTCACATTTCATCCTCCTG 1348 (M94157) This study
T150-Quasar670 AACCAATCTCTATGTGGACTACATACCA 1225 (M94157) Chun et al., 2010
PAstV1 (capsid) T217-F CCAAAACCAGCAATCCGTCAA 260 (Y15938) This study
T218-R GCCCCTAAAGCAACGATCGG 420 (Y15938) This study
T219-Quasar705 TTCTTGTCAAGGATAATACGGGG 363 (Y15938) This study
PAstV4 (RdRp) T220-F ACAGCGCTGCATGGGAAACTC 863 (GU562296) This study
T221-R AGGCTTATGCTTTGGTCCGC 1045 (GU562296) This study
T222-FAM AGGCAGATGGACAGGCTTTGGAG 1001 (GU562296) This study
PKoV (5′UTR) T248-F TCTCTGACCTCTGAAGTGCACT 462 (JX401523.1) This study
T249-R TGAAGAAGCCATGTGTCTTGTC 589 (JX401523.1) This study
T250-FAM GGTTGCGTGGCTGGGAATCCAC 486 (JX401523.1) This study
PCV2 (Rep) T176-F GGCCACCTGGGTGTGGTAAA 571 (JQ002672) Gagnon et al., 2010
T177-R CCCACCACTTGTTTCTAGGTGGTT 660 (JQ002672) Gagnon et al., 2010
T178–FAM TTTGCAGACCCGGAAACCACATACTGGA 609 (JQ002672) Gagnon et al., 2010
MS2 T210-F TGGCACTACCCCTCTCCGTATTCAC 289 (NC.001417) Liu et al., 2011a
T211-R GTACGGGCGACCCCACGATGAC 387 (NC.001417) Liu et al., 2011a
T212-TET CACATCGATAGATCAAGGTGCC 330 (NC.001417) Liu et al., 2011a

Note: (+C) or (+T) indicates a locked nucleic acid (LNA).