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. 2010 Aug 21;24(6):415–417. doi: 10.1016/j.mcp.2010.08.003

Fig. 1.

Fig. 1

Sensitivity (A) and specificity (B) of RT-LAMP assay evaluated by eye after the addition of hydroxynaphthol blue dye. A) Lanes 1–6: cDNA from the TCoV (TCoV/BR/2007) at different titers: 104 EID50/50μl (1), 103 EID50/50μl (2), 102 EID50/50μl (3), 101 EID50/50μl (4), 100 EID50/50μl (5), 10−1 EID50/50μl (6); lane 7 corresponded to water instead of viral cDNA; B) Lanes 1–6: RNA from TCoV (TCoV/BR/2007) (1), NDV (2), TAstV-1 (3), IBV-M41 (4), IBV-H120 (5), AMPV (6); C) Agarose gel electrophoresis of RT-LAMP products from 102 EID50/50μl (line 2), 103 EID50/50μl (line 3) dilution of TCoV; line 1, DNA marker 2 kb plus; D) Sensitivity and specificity of conventional RT-PCR; Lanes (1) corresponded to water instead of viral RNA, (2) TAstV-1, (3) IBV-41, (4) IBV-H120; Lanes 5–10: RT-PCR products from TCoV at different titers: 10−1 EID50/50μl (5), 100 EID50/50μl (6), 101 EID50/50μl (7), 102 EID50/50μl (8), 103 EID50/50μl (9), 104 EID50/50μl (10); Lanes 11–13 tissue corresponded to RT-PCR amplification from RNA extracted from uninfected poult embryos.