Fig. 2.

Characterization of the SLIM system for pathogen enrichment. (A) Amplification efficiency for a 1 mL E. coli test sample (10⁴ CFU) following its enrichment on the SLIM system using the DMA HI reagent. The black dotted line represents the C_T value for E. coli DNA from the absolute concentration (10⁴ CFU) reference control extracted with a Qiagen kit. (B) The E. coli capture rate using the SLIM system depends on the HI concentration. (C) The amplification efficiency following E. coli (10⁴ CFU/mL) enrichment depends on the incubation time on the platform. The black dotted line denotes the C_T value of the absolute concentration (10⁴ CFU) E. coli reference control DNA extracted using a Qiagen kit. (D) The E. coli capture rate using the SLIM system also depends on the incubation time. The error bars indicate the standard deviation from the mean, based on at least three independent experiments.