Fig. 3.
Application of the SLIM system to bacterial enrichment and extraction. (A) The amplification efficiency for a sample of E. coli (104 CFU/mL) following bacterial enrichment using the SLIM system is dependent on the type of HI reagents (DMA; yellow, DMP; blue, DMS; purple). The control experiment without HI reagents is examined as negative control (No HI). The black dotted line denotes the CT value for the reference control E. coli DNA extracted from the absolute concentration (104 CFU) sample control of using a Qiagen kit. (B) The E. coli capture rate using the SLIM system depends on the HI concentration. The error bars indicate the standard deviation from the mean, based on at least three independent experiments. (C) Capacity of the SLIM system to process test E. coli samples in concentrations ranging from 1 × 101 to 1 × 104 CFU/mL using both the Qiagen kit (red) and the SLIM system (light green) for DNA extraction. The error bars indicate the standard deviation from the mean, based on at least three independent experiments. (D) Use of the SLIM system for simultaneous enrichment and extraction with a test E. coli sample of 1 × 104 CFU/mL. Qiagen (light gray); DNA extraction only without enrichment, Two-step (dark gray); DNA extraction with Qiagen kit after bacterial enrichment with the SLIM platform, and One-step (black); simultaneous enrichment and extraction on the same system. The black dotted line denotes the CT value of the absolute (104 CFU) E. coli reference control DNA extracted using a Qiagen kit. The error bars indicate the standard deviation from the mean, based on at least three independent experiments. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).