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. 2009 Nov 3;29(4):695–713. doi: 10.1016/j.cll.2009.07.010

Table 1.

Nucleic acid amplification methods and primer sets used for detection of human bocavirus 1

Target Gene Target Locationa Predicted Size (bp) Forward Primer Reverse Primer Probeb % Detectedc (Mediand) References
Polymerase chain reaction Conventional NP1 2351–2704 353 GACCTCTGTAAGTCTATTAC CTCTGTGTTGACTGAATACAG 0.8–45.2 (5.4) 11, 14, 20, 24, 28, 29, 34, 38, 39, 40, 47, 48, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 83, 102
NS1 2301–2700 399 CCCAAGAAACGTCGTCTAAC GTGTTGACTGAATACAGTGT 2.8–2.9 (2.90) 62, 63, 76, 103, 104, 105
VP1/VP2 1545–1835 290 TATGGCCAAGGCAATCGTCCAAG GCCGCGTGAACATGAGAAACAGA 0.8–11.3 (5.5) 35, 40, 41, 42, 43, 49, 56, 59, 60, 77, 102
VP1/VP2 4125–4966 841 GTGACCACCAAGTACTTAGAACTGG GCTCTCTCCTCCCAGTGACAT 5.1–12.5 (8.8) 24, 47, 66, 74
VP1/VP2 4370–5189 819 GGACCACAGTCATCAGAC CCACTACCATCGGGCTG 3.5–5.5% (4.50) 42, 43
VP1/VP2 3639–4286 647 TTCAGAATGGTCACCTCTACA CTGTGCTTCCGTTTTGTCTTA 6.60 65
VP1/VP2 4562–4966 404 GCAAACCCATCACTCTCAATGC GCTCTCTCCTCCCAGTAGACAT 1.5–14.2 (7.6) 14, 24, 47, 66, 74
VP1/VP2 4268–4467 199 AGACAAAACGGAAGCACAGC TCAAAGCCAGATCCAAATCC 3.10 84
VP1/VP2 4269–4467 198 CAGTGGTACCAGACACCAGAAG GCCAGTTCTTTGTTGCGTATCT 5.5–31.3 (14.0) 25, 84
VP1/VP2 4270–4467 197 CCAAAAAAGACACTTTTACTTTGCTAACTCA TGGACGCCAGTTCTTTGTTGCGTATCTTTC 2.2–2.7 (2.40) 48
Nested NP1 2259–3259 1000 Outer: GAAGACACCGAGCCTGAGAC Outer: GCTGATTGGGTTGTTCCTGAT 7.10 106
2351–2565 214 Inner: GAGCTCTGTAAGTACTATTAC Inner: ATATGAGCCCGAGCCTCTCT
Paired Nestede NP1 2318–2709 391 Outer: TAACTGCTCCAGCAAGTCCTCCA Outer: GGAAGCTCTGTGTTGACTGAAT 7.4–11.1 (9.3) 107, 108
2344–2709 365 Inner: CTCACCTGCGAGCTCTGTAAGTA Inner: GGAAGCTCTGTGTTGACTGAAT
VP1/VP2 4085–4988 903 Outer: GCACTTCTGTATCAGATGCCTT Outer: CGTGGTATGTAGGCCGTGTAG
4139–4988 849 Inner: CTTAGAACTGGTGAGAGCACTG Inner: CGTTGGTATGTAGGCGTGTAG
NS1 1579–1794 215 Outer: TATGGGTGTGTTAATCATTTGAAYA Outer: GTAGATATCGTGRTTRGTKGATAT 5.80 50
1600–1703 103 Inner: AACAAAGGATTTGTWTTYAATGAYTG Inner: CCCAAGATACACTTTGCWKGTTCCACCC
NS1 2325–2723 398 Outer: CCAGCAAGTCCTCCAAACTCACCTGC Outer: GGAGCTTCAGGATTGGAAGCTCTGTG
2351–2704 353 Inner: GAGCTCTGTAAGTACTATTAC Inner: CTCTGTGTTGACTGAATACAG
Real-time NP1 22548–2628, 2570 AGAGGCTCGGGCTCATATCA CACTTGGTCTTGAGGTCTTCGAA AGGAACACCCAATCARCCACCTATCGTCT 2.9–32.3 (9.1) 21, 36, 85
NP1 2509–2605, 2534 AGGAGCAGGAGCCGCAGCC CAGTGCAAGACGATAGGTGGC ATGAGCCCGAGCCTCTCTCCCCACTGTGTC g 22
NP1 2397–2592, 2533 CCACGTGACGAAGATGAGCTC TAGGTGGCTGATTGGGTGTTC CCGAGCCTCTCTCCCCACTGTGTCG 11.9 78
NP1 2405–2586, 2506 CGAAGAATGAGCTCAGGGAAT GCTGATTGGGTGTTCCTGAT CACAGGAGCAGGAGCCGCAG 9.0–11.3 (10.7) 17, 51, 67, 86
NP1 2427–2580, 2504 GAAAGACAAGCATCGCTCC TGGGTGTTCCTGATGATATG CGCGCTGGAGCAGGAGCCGCAGCCCGATAGCGCG 2.9 87
NP1 2351–2704, 2480 GAGCTCTGTAAGTACTATTAC CTCTGTGTTGACTGAATACAG GGAAGAGACATGGCAGACAAC 12.8 79
NP1 2391–2466, 2411 GCACAGCCACGTGGCAGACAA TGGACTCCCTTTTCTTTTGTAGGA TGAGCTCAGGGAATATGAAAGACAAGCATCGTMR 4.6 44
NP1 2480–2579, 2504 GGAAGAGACACTGGCAGACAA GGGTGTTCCTGATGATATGAGC CTGCGGCTCCTGCTCCTGTGAT 2.4–18.9 (5.3) 15, 26, 30, 88
NP1 2424–2636, 2559 TATGAAAGACAAGCATCGCTCCTA GTCTTCATCACTTGGTCTGAGGTCT CTCATATCATCAGGAACAC 8.2 37
NP1 2554–2628, 2577 TCGGGCTCATATCATCAGGAA CACTTGGTCTGAGGTCTTCGAA CCCAATCAGCCACCTATCGTCTTGC 19.3 83
NP1 2488–2566, 2512 CACTGGCAGACAACTCATCACA GATATGAGCCCGAGCCTCTCT AGCAGGAGCCGCAGCCCGA 4.5 89
NP1 1617–1710, 1667 TAATGACTGCAGACAACGCCTAG TGTCCCGCCCAAGATACACT TTCCACCCAATCCTGGT g 90
NP1 1637–1711, 1668 TAGTTGTTTGGTGGGARGA CTGTCCCGCCCAAGATACA CCAGGATTGGGTGGAACCTGCAAA 9.1 54
NP1 1624–1711, 1668 TGCAGACAACGCYTAGTTGTTT CTGTCCCGCCCAAGATACA CCAGGATTGGGTGGAACCTGCAAA 2.9–32.3 (3.5) 36, 42, 85
NASBAf NP1 2567–2424, 2456 AATTCTAATACGACTRCACTATAGGGGCTGATTGGGTGTTCCTGAT AATTCTAATACGACTCACTATAGGGCGAAGATGAGCTCAGGGAAT CGATCGAGTCCAGAAAGAGGGGAGAGCGATCG 0 94
a

Estimated location based on GenBank isolate EU262978.1, probe location added when applicable.

b

Added when applicable.

c

Range of percent detected in cited studies.

d

Median of percent detected in cited studies when applicable.

e

In cited studies, a sample positive for HBoV-1 required amplification from both sets of nested PCR primers.

f

Nucleic acid sequence based amplification.

g

Case reports.