Table 1.
Reactivity of N protein-EGFP constructs with anti-N monoclonal antibodies
| mAb | Epitopeb | Reactivity with EGFP constructsa |
||||||
|---|---|---|---|---|---|---|---|---|
| No peptide | 43–72 | 1–123 | 1–123 G43,G44 | 1–123 G46,G47 | 14–123 | 1–115 | ||
| SDOW-17 | 30–52, 112–123 | − | − | + | + | + | + | + |
| SR-30 | 69–123 | − | − | + | + | + | + | − |
| MR-40 | 52–69, 112–123 | − | − | + | + | + | + | + |
| EP-147 | 52–69, 112–123 | − | − | + | + | + | ± | NDc |
| JP-24 | 52–69, 112–123 | − | − | + | + | + | + | + |
| VO-17 | 52–69, 112–123 | − | − | + | + | + | + | − |
a
Peptide region of N protein fused to EGFP.
b
The epitope regions recognized by each monoclonal antibody (mAb) are described in Wootton et al. (1998) and are identified by beginning and ending amino acid.
c
ND, not determined.