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Small molecule inhibitors affect DUBs in living cells; (A) Workflow of DUB inhibition profile in living cells revealed by activity-based quantitative mass spectrometry as described in [51]. (B) Workflow of profiling inhibitor capacity by immunoblot as described in [51]. (C) HEK293T cells were incubated with 50 µM PR-619, P22077, 0.1% DMSO or 10 µM MG132, lysed and analyzed by SDS-PAGE and anti-HA immunoblotting.
