Fig. 4.

PSV enters PK-15 cells occurs in a dynamin-dependent manner. (A) PK-15 cells were pretreated by 10 μM dynasore for 1 h and incubation with 50 μg/ml Alexa Fluor 568-conjugated transferrin for 60 min. Cells were then fixed and analyzed by confocal microscope. Bars, 30 µm. (B) Cells were mock-treated or pre-treated with dynasore for 1 h and infected with PSV (MOI = 2) or dynasore was post treated after virus added for 1 h. At indicated time, cells were processed for western blot and quantification of the bands corresponding to VP1 was corrected with α-tubulin. Data was then normalized to the value from mock-treated cells. (C) Similar to above, after cells were treated with different concentrations drugs and infected with PSV. Cell lysates were collected and virus titers or viral RNA copy number were determined. (D) The effect of wild-type dynamin (Dyn WT) and dominant negative mutant of dynamin (Dyn DN) on PSV infection was determined by western blot as described above. Data are presented as means ± SD from at least two independent experiments. ^*P < 0.05, ^**P < 0.01 compared to the mock-treated cells.