Fig. 6.

PSV enters PK-15 cells leads to active actin rearrangement. (A) PK-15 cells were mock-treated or infected with PSV (MOI = 5) for 60 min and then fixed, stained for actin by Alexa Fluor 594-phalloidin (red) for 30 min, followed by staining with anti-VP1 antibody, Alexa Fluor-488-conjugated goat anti-mouse secondary antibody (green) and DAPI (blue). Bars, 30 µm. (B and C) The effect of actin inhibitor Cytochalasin D (CytoD), and Jasplakinolide (Jasp) on virus VP1 protein expression detected by western blot analysis. (D and E) Effects of pretreatment of PK-15 cells with increasing concentrations of CytoD or Jasp on PSV particle yield, as detected by virus titers assay. Data are means ± SD from at least two independent experiments. ^*P < 0.05, ^**P < 0.01 compared to the mock-treated cells.