Table 1.

Primer sequences used for RT-PCR and sequencing of the ORF1 gene.

Oligonucleotide	Orientation	Nucleotide sequence	Locationa	Length	Reference
P290d	Forward	5′-GATTACTCCASSTGGGAYTCMAC-3′	4445	319	Pinto et al. (2012)
P289d	Reverse	5′-TGACGATTTCATCATCMCCRTA-3′	4763
FNoV5f	Forward	5′-AACACTGCCTACGTCCAACC-3′	89	746	Pinto et al. (2012), JF781268b
FNoV5r	Reverse	5′-CAGGACTGGTAGGGGTCGTA-3′	834
FNoV3f	Forward	5′-CTTTTTGCTGCCAAGTCCTC-3′	741	1736	Pinto et al. (2012), JF781268b
FNoV3r	Reverse	5′-CACCTGATCTTGGACCCTGT-3′	2476
FNoV8f	Forward	5′-GCCTTCATCAAAACCCTTGA-3′	1424	2085	Pinto et al. (2012), JF781268b
FNoV8r	Reverse	5′-ACCCTGCACCTTCATAGTGG-3′	3508
FNoV2f	Forward	5′-CCTCCATCTGGTCGAGGATA-3′	3183	1696	Pinto et al. (2012), JF781268b
FNoV2r	Reverse	5′-GAGAGTTCCCTGCTGACGAC-3′	4878
FNoV/CNoV10f	Forward	5′-GTTGCAGCAAGTGATGCGGG-3′	3892	1948	Pinto et al. (2012), Martella et al. (2009), JF781268b, FJ875027b
FNoV/CNoV10r	Reverse	5′-CAGGACACCGTGAACACATC-3′	5839

^aThe oligonucleotide locations were referred to the full-genome sequence of the FNoV CU81210E strain (JF781268; Pinto et al., 2012).

^bGenBank accession number.