Table 2.
Primer sequences used for RT-PCR and sequencing of ORF2 and 3 genes.
| Oligonucleotide | Orientation | Nucleotide sequence | Locationa | Length | Reference |
|---|---|---|---|---|---|
| CNoVGVI22f | Forward | 5′-GCTTGAGCCCATAGTCTTGC-3′ | 1244 | 998 | Martella et al. (2009), FJ875027b |
| CNoVGVI22r | Reverse | 5′-AACCTTGAGTTGGACGTTGG-3′ | 1939 | ||
| CNoVGVI23f | Forward | 5′-CCAACGTCCAACTCAAGGTT-3′ | 1920 | 696 | Martella et al. (2009), FJ875027b |
| CNoVGVI23r | Reverse | 5′-AGTTGAGAGCTGGGTGGAGA-3′ | 2710 |
The oligonucleotide locations were referred to the genome sequence of the CNoV Bari-91 strain (FJ875027; Martella et al., 2009).
GenBank accession number.