Fig. 2.

Verification of ALI model and measurement of relevant proteins.

A. TNFα, IL-1β, and IL-10 in BEAS-2B cells were examined using ELISA 12 h, 24 h or 48 h after treatment of 10 mg/ml LPS. The vertical coordinate indicates the concentration of target protein, and the horizontal coordinate indicates the experiment group. B. PAF and AA contents in BEAS-2B cells were measured using ELISA 12 h, 24 h or 48 h after treatment of 10 mg/ml LPS. The vertical coordinate indicates the concentration of target protein, and the horizontal coordinate indicates the experiment group. C. CDK2, CCSP1 and PLA2 expression levels, as well as C/EBP β phosphorylation, were assessed by western blotting 12 h, 24 h or 48 h after treatment of 10 mg/ml LPS. Lower: image of target blots, upper: analysis on the optical density of three proteins, β actin serving as the reference, and for the phosphorylated protein, the total protein of itself serving as the reference. Data are means ± SD from at least three separate replicates.*, p < 0.05; **, p < 0.01. vs cell group.