Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 29;629:76–85. doi: 10.1016/j.gene.2017.07.076

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Elsevier B.V. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 4 — Verification of C/EBP β binding site on CCSP1 promoter.

A. BEAS-2B cells were infected with the indicated lentivirus, and CCSP1 mRNA was measured by real time quantitative PCR 72 h later. The vertical coordinate indicates the relative amount of CCSP1 mRNA and the horizontal coordinate indicates the experiment group, β actin serving as the reference. B. Verification of C/EBP β binding site on CCSP1 promoter by CHIP-PCR. Upper: relatively quantitative analysis on DNA segments, the vertical coordinate being the ratio of gene copy number of the sample to the control gene copy number of Input, and the horizontal coordinate being experiment group. Lower: western blotting results of C/EBP β protein, Input is the negative control without antibody, and IgG is the control group with a primary antibody against GAPDH. C. Measurements of luciferase activity. The vertical coordinate indicates the luciferase activity expressed as the firefly luciferase/Renilla luciferase, and the horizontal coordinate indicates the experiment group. The determination of the luciferase activity was made 48 h after transfection of 293 cells. Data are means ± SD from at least three separate replicates. *, p < 0.05; **, p < 0.01.