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. 2006 Oct 12;14(24):8295–8306. doi: 10.1016/j.bmc.2006.09.014

Figure 2.

Figure 2

Characterization studies of SARS-CoV 3CLpro and SARS-CoV 3CLpro Q189A proteases. (A) CD spectra of SARS-CoV 3CLpro and SARS-CoV 3CLpro Q189A Far-UV CD spectra of SARS-CoV 3CLpro (–■–) and SARS-CoV 3CLpro Q189A (–○–) at 25 °C. Protein concentration used in CD experiments is 10 μM, and the two protein samples were prepared in 20 mM Tris–HCl, pH 7.4, 120 mM NaCl. (B) Fluorescence spectra of SARS-CoV 3CLpro and SARS-CoV 3CLpro Q189A fluorescence spectra of SARS-CoV 3CLpro (—) and SARS-CoV 3CLpro Q189A (…) at 25 °C. Protein concentration used in the experiment is 10 μM, and two protein samples were prepared in 20 mM Tris–HCl, pH 7.4, 120 mM NaCl.