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. 2006 Oct 12;14(24):8295–8306. doi: 10.1016/j.bmc.2006.09.014

Figure 3.

Figure 3

Kinetic parameters determination of SARS-CoV 3CLpro and SARS-CoV 3CLpro Q189A. The enzymatic activity of SARS-CoV 3CLpro and SARS-CoV 3CLpro Q189A was measured by the fluorescence resonance energy transfer (FRET) method. SARS-CoV 3CLpro or SARS-CoV 3CLpro Q189A (1 μM) and the fluorogenic substrate with different concentrations from 5 to 200 μM were incubated at 25 °C. The fluorescence intensity was monitored continuously for 1 h. The Km and kcat values were calculated by Lineweaver–Burk plot. (A) The double reciprocal plot of SARS-CoV 3CLpro, (B) the double reciprocal plot of SARS-CoV 3CLpro Q189A.