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. 2006 Oct 12;14(24):8295–8306. doi: 10.1016/j.bmc.2006.09.014

Figure 4.

Figure 4

Binding affinity of quercetin-3-β-galactoside to SARS-CoV 3CLpro and SARS-CoV 3CLpro Q189A determined by SPR. Specificity and concentration-dependent binding and real-time binding affinity measurement of quercetin-3-β-galactoside to immobilized SARS-CoV 3CLpro and SARS-CoV 3CLpro Q189A (A and B) using Biacore 3000. For SARS-CoV 3CLpro, the concentrations of quercetin-3-β-galactoside were 80, 56, 39.2, 27.4, 19.2, 13.4, and 9.4 μM, while for SARS-CoV 3CLpro Q189A, the concentrations of quercetin-3-β-galactoside were 200, 140, 98, 68.6, 48.0, 33.6, 23.5, and 16.5 μM from top to bottom. Quercetin-3-β-galactoside was injected for 60 s, and dissociation was monitored for more than 120 s, respectively.