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Enzyme kinetic analysis of SARS-CoV 3CLpro by quercetin-3-β-galactoside. The reaction was performed in the presence of different concentrations of quercetin-3-β-galactoside (▴, 0 μM; ▵, 10 μM; ○, 50 μM; •, 100 μM) at varying concentrations of the fluorogenic substrate (from 5 to 100 μM). Lineweaver–Burk plots of kinetic data were fitted to obtain the Ki value of 35.66 ± 4.57 μM.
