Fig. 4.

(a) Different flow rates at 1, 4, 7 and 10 μL/min at room temperature while keeping Cy3-labeled target DNA volume constant at 6 μL. The concentration of target DNA is 50 pM and the height of microfluidic flow channel is 50 μm. Fluorescence signals in the first row correspond to 1 μL/min, 4 μL/min in the second row, 7 μL/min in the third row and 10 μL/min in last row. Note that there is no indication of binding at the Corona Virus DNA sites, the control or inside the ellipses. (b) Fluorescence intensity vs. a flow rate of 50 pM of target DNA in the hybridization solution. The number of experiments done (n) is equal to 6.