Fig. 3.

Sedimentation rate of virus inhibitor from lamb brain tissue in a discontinuous sucrose gradient. Sucrose solutions in PBS, 5 mM MgC1₂, were used to form step gradients. Equivalent volumes of brain inhibitor preparation (0.5 ml) and 50% sucrose were mixed, layered on the discontinuous gradient, and centrifuged at 31 000 rev./min in a SW-55 rotor for 90 min. Collected fractions were examined for antiviral activity against Sindbis virus after extensive dialysis. Reprinted with permission from Antiviral Res., 1995, 27:380.