Fig. 5.

Characterization of a homotypic N protein interaction in an intracellular genomic RNP complex. Coinfected cells were radiolabeled and cell extracts were prepared using lysis buffer. Intracellular genomic RNP complex was immunoprecipitated with anti-M monoclonal antibody, and then released from the antigen-antibody complexes by high-salt buffer treatment of the immunoprecipitates. One-half of the intracellular genomic RNP complex was incubated with RNase A (lanes 4–6), while the other half was mock-treated (lanes 1–3). After RNase treatment, the samples were immunoprecipitated with anti-N protein monoclonal antibody J3.3. (lanes 1, 4), preimmune serum (lanes 2, 5) or anti-N-spacer antibody (lanes 3, 6), and analyzed by SDS–PAGE.