Table 1.
Summary of pig groups, inoculum and diarrhea outcomes after infection with PDCoV inpigs.
| Groups | Pig numbers | Inoculuma | Calculated inoculum infectious titer (log10 TCID50/mL)c | Tested inoculum RNA titer (CT value)d | Diarrhea (percent)e | Fecal viral RNA shedding (CT value) |
|---|---|---|---|---|---|---|
| G1 | 5 | original P6b | 4.0 | 13.34 | 3 (5/5, 100) | 13.43−28.51 |
| G2 | 5 | 10−1 diluted P6 | 3.0 | 15.21 | 2−3 (5/5, 100) | 15.18−28.78 |
| G3 | 5 | 10−2 diluted P6 | 2.0 | 17.60 | 0−1 (0/5, 0) | - f |
| G4 | 5 | 10−3 diluted P6 | 1.0 | 23.61 | 0 (0/5, 0) | - f |
| G5 | 5 | PBS | – | – | 0 (0/5, 0) | - f |
a
All pigs were orally inoculated with 3 mL of inoculum.
b
G1 pigs were infected with undiluted cell culture-adapted CH/XJYN/2016 P6.
c
Titers were calculated based on the titer of the original P6 virus (original titer: 104.0 TCID50/mL) and dilution times.
d
CT value: the mean cycle threshold value. A cutoff point was set at 30; CT values greater than 30 were considered negative.
e
Fecal scores were determined during the 7 day postinfection (dpi) period for pigs in all groups. Scores for fecal consistency: 0 = normal; 1 = pasty; 2 = semiliquid; and 3 = liquid.
f
Negative or below the detection limit of real-time PCR.